For the DEA first you must choose the Number of Genes to be plotted per cluster (maximum 8) (1) and the Statistical Method to be employed in the analysis (Wilcoxon or t-test) (2). In (3) you can choose a custom Gene List to plot. After that, click on Run (4) to start the analysis. Above (5) will appear a link named Differential Expression Analysis that will send the user to the full DEA results per cluster and below (6) two options for Dot Plot will be generated. Here, the color indicates whether the gene is up- or down-regulated and the dot size indicate the percentage in which each gene is expressed at each cluster. Next, click on Continue to Visualization (7) to plot genes in UMAP or PCA.
